Somatic cell hybridization was used extensively to develop novel cytoplasmic recombinants (Fig.2.). Somatic hybrids were produced involving mustard and several of its wild allies including Brassica spinescens, Moricandia arvensis, Trachystoma ballii, Diplotaxis catholica, Erucastrum laevigatum. Sinapis alba, Diplotaxis erucoides, Brassica cossoneana, E. rucastrum abyssinicum and E. gallicum. Several cytoplasmic male sterility systems of mustard have been developed by combining the cytoplasm of wild relatives such as B.oxyrhina, D.catholica, T.ballii, M.arvenis etc. with the nucleus of mustard through repeated backcrossing of respective somatic hybrids with mustard. M.arvensis - based CMSand restores lines have been distributed to the breeders for developing commercial hybrids.
Contact: Dr. S.R. Bhat ( srbhat22@rediffmail.com )
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