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Sequencing of 8.2 million base pairs of Rice Genome |
International Rice Genome Sequencing Project (IRGSP) is a consortium of ten countries namely, Brazil, China, France, India, Japan, Korea, Taiwan, Thailand, UK and USA. By becoming member of the IRGSP, India for the first time participated in any genome sequencing project. The NRCPB is one of the two centers involved in the Indian Initiative for the Rice Genome Sequencing (IIRGS), the other one being Delhi University South Campus. The responsibility of NRCPB was to sequence rice genome from the long arm of chromosome 11 between map positions 57.3 cM to 84.3 cM and furthermore to identify genes and markers of agricultural importance using the rice genome sequence data. Summary of the total sequence submitted to GenBank and number of genes predicted is given in the Table. |
| Total Rice genome sequences submitted in the GenBank |
8176601 bp |
| Total no. of gene predicted |
810 |
| Putative genes |
252 |
| Unknown genes |
201 |
| Hypothetical genes |
357 |
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| Chromosome sharing in IRGSP |
| Contact : Dr.N.K.Singh ( nksingh@nrcpb.org ) |
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High resolution mapping and cloning of Pi-kh gene from rice |
The center is actively involved in developing DNA markers tightly linked to the disease resistance genes in rice and mustard. Two closely linked SSR markers, TRS 26 and TRS 33 were mapped at 0.7 cM and 0.5 cM flanking to Pi-kh gene, respectively on long arm of chromosome 11. The STMS marker was also mapped at a distance of 0.7 cM. The closely linked SSR markers developed during this study can be used for marker-assisted selection in pyramiding Pi-kh gene along with other blast resistance genes. Map based cloning of Pi-kh locus from rice line Tetep has been accomplished. The analysis of translated product of ORF showed that this gene belong to the Nucleotide Binding Site (NBS), Leucine Rich Repeat (LRR) class of the R-genes. |
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| Contact : Dr. T. R. Sharma ( trsharma@nrcpb.org ) |
| Development of Molecular Marker for the White Rust Resistance Gene in Mustard |
RFLP and RAPD linkage maps have been used to tag genes for yellow seed coat colour and resistance to white rust caused by Albugo candida respectively. These markers are flanking the target genes and thus are useful in Marker Aided Selection (MAS). Particularly, for the white rust resistance locus Ac2 (t), reproducible PCR based SCAR/CAPS markers have been developed for carrying out MAS. |
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Cleaved Amplified Polymorphic DNA (CAPS) marker developed for the White Rust Resistance Gene in Inidan mustard for use in Marker Aided Selection (MAS) |
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| Contact : Dr. T. Mohapatra
( tm@nrcpb.org ) |
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DNA fingerprints for Wheat, Rice and Brassica generated |
| DNA profiling systems such as RFLP, RAPD, STMS, ISSR, ASSR and AFLP were standardized for variety identification in rice including the Basmati varieties, wheat and mustard. A database of DNA fingerprints has been created for 120 cultivars in rice, 140 cultivars in wheat and 42 cultivars in Brassica , which provides molecular descriptors for variety identification. This is going to be utilized as National Reference for variety registration and protection. |
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| Fig.1 Dendrogram of wheat cultivars based on STMS markers |
Fig.2 A segment of AFLP profiles of 31 Indian wheat varieties |
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| Development of new STMS markers for sugarcane |
Contact : Dr. N.K.Singh ( nksingh@nrcpb.org ) |
