Research Interests :
The work in Bt Laboratory is focused on isolation and characterization of novel Bt ( Bacillus thuringiensis ) genes, protein engineering of δ-endotoxins in E. coli , expression in transgenic plant systems and development of efficient plant transformation techniques such as gene targeting.
Selected Publications:
- Rajam, M V. Prabhavati V, Rotino G L, Sihachakr D, Mansur E and Kumar, P. A. (2008) Eggplant. In: Kole C, Hall TC (eds). “Compendium of Transgenic Crop Plants: Transgenic Vegetable Crops”, Blackwell Publishing, Oxford, UK, 2008, pp 47-72.
- Chen, Z. J., ----, Kumar, P. A., ---- and Paterson, A.H. (2007). Towards sequencing cotton (Gossypium) genomes. Plant Physiology 145: 1303-1310.
- Dalal M., Dani R. G. and Kumar P. A. (2006). Current trends in the genetic engineering of vegetable crops Scientia Horticulturae 107: 215-225.
- Aziz, M. A, Sikriwal, D., Singh, S., Jarugula, S. and Kumar, P. A.; Bhatnagar, R. (2005). Transformation of an edible crop with pag E gene of Bacillus anthracis. FASEB Journal 19: 1501-03.
- Bhalla, R., Dalal, M., Panguluri, S K., Jagadish, B., Mandaokar, A., Singh, A. K., and Kumar, P. A. (2005). Isolation, characterization and expression of a novel vegetative insecticidal protein gene of Bacillus thuringiensis. FEMS Microbiology Letters 243: 467-472.
- Kumar, P. A. (2003) Insect pest-resistant transgenic crops. In: Advances in Microbial Control of Insect Pests (Ed. Upadhyay, R K.), pp. 71-82, Kluwer Academic, New York.
- Mandaokar, A., Goyal, R. K., Shukla, A., Bhalla, R., Chaurasia, A., Reddy, V. S., Altosaar, I., Sharma, R. P. and Kumar, P. A. (2000) Transgenic tomato plants resistant to fruit borer ( Helicoverpa armigera Hubner). Crop Protection 19: 307-312.
- Chandra, A., Ghosh, P., Mandaokar, A., Bera, A K., Sharma, R P., Das, S. and Kumar, P. A.. (1999) Amino acid substitution in α-helix 7 of Cry1Ac δ-endotoxin of Bacillus thuringiensis leads to enhanced toxicity to Helicoverpa armigera Hubner. FEBS Letters 458: 175-179.
- Kumar, P. A., Mandaokar, A., Sreenivasu, K., Chakrabarti, S. K., Sharma, S. R., Bisaria, S., Kaur, S. and Sharma, R P. (1998). Insect-resistant transgenic brinjal plants. Molecular Breeding 4: 33-37.
- Kumar, P. A., Malik, V. S. and Sharma, R P. (1996) Insecticidal proteins of Bacillus thuringiensis . Advances in Applied Microbiology 42:1-43.
Email: kumarpa@nrcpb.org |